Gelatin-coated magnetic nanoparticles-based DNA isolation method: A comparison with commercial DNA isolation kits from whole blood
نویسندگان
چکیده
منابع مشابه
Comparison of commercial DNA extraction kits for isolation and purification of bacterial and eukaryotic DNA from PAH-contaminated soils.
Molecular characterization of the microbial populations of soils and sediments contaminated with polycyclic aromatic hydrocarbons (PAHs) is often a first step in assessing intrinsic biodegradation potential. However, soils are problematic for molecular analysis owing to the presence of organic matter, such as humic acids. Furthermore, the presence of contaminants, such as PAHs, can cause furthe...
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Blood is the main source of DNA for genotype-related studies in humans. A rapid, efficient, and cost-effective method for the isolation of genomic DNA from whole blood is needed for screening a large number of samples. There are many published protocols (1,3–7). These procedures use toxic organic chemicals (SDS-proteinase Kphenol isolation method or its modifications) (1,3) and/or are time cons...
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A number of methods have been described for the isolation of genomic DNA from whole blood. However, these described methods are either unreliable or too expensive to be used routinely in small diagnostic laboratories of developing countries. We have evaluated the slightly modified salt-out method for DNA isolation so that it can be applied on whole blood samples using a three step procedure: (1...
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Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods--namely three DNA isolation protocols and three commercial DNA isolation kits--...
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Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extraction of the supercoiled (sc) form of plasmid DNA (pDNA) from agarose gel. These MNPs were attached with 5'-NH(2)-tagged oligo-(dT)(25) primer and were used to isolate mRNA from breast cancer cells. The isolated mRNA was used for amplificatio...
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ژورنال
عنوان ژورنال: Journal of Bioscience and Applied Research
سال: 2019
ISSN: 2356-9182
DOI: 10.21608/jbaar.2019.138335